Characterization of 14 Microsatellite Markers for Silene acaulis (Caryophyllaceae)
نویسندگان
چکیده
منابع مشابه
Characterization of 14 microsatellite markers for Silene acaulis (Caryophyllaceae)1
PREMISE OF THE STUDY Fifty candidate microsatellite markers, generated using 454 shotgun sequencing, were tested for the widespread arctic/alpine herb Silene acaulis (Caryophyllaceae). METHODS AND RESULTS Fourteen out of 50 markers resulted in polymorphic products with profiles that enabled interpretation. The numbers of alleles per locus ranged from two to six, and the expected heterozygosit...
متن کاملDevelopment of highly variable microsatellite markers for the tetraploid Silene stellata (Caryophyllaceae)1
PREMISE OF THE STUDY We designed and tested microsatellite markers for the North American native species Silene stellata (Caryophyllaceae) to investigate its population genetic structure and identify selection on floral design through male reproductive success. METHODS AND RESULTS A total of 153 candidate microsatellite loci were isolated based on next-generation sequencing. We identified 18 ...
متن کاملin Silene latifolia (Caryophyllaceae)
• Sex ratio polymorphism has been extensively studied in Silene latifolia, but it is neither known whether inbreeding (which is likely to occur under field conditions) affects it nor which of the proposed mechanisms (Y degeneration, X-linked drive) is more important. Both mechanisms predict reduced pollen performance. • In this study, females were crossed with pollen from related and unrelated ...
متن کاملLife history of the long-lived gynodioecious cushion plant Silene acaulis (Caryophyllaceae), inferred from size-based population projection matrices.
Alpine plants often appear to have long life-spans as an adaptation to harsh and unpredictable environmental conditions, yet many lack reliable indicators of age that would make it possible to determine their true longevity. Their extended life-spans also pose problems for measuring lifetime reproductive success, a key component of breeding system evolution in species such as the gynodioecious ...
متن کاملDevelopment and characterization of 10 microsatellite markers in Sagina nodosa (Caryophyllaceae)1
UNLABELLED PREMISE OF THE STUDY We developed 10 novel microsatellite loci for Sagina nodosa, a diploid perennial arctic-alpine herb. To our knowledge, these are the first microsatellite loci for a Sagina species. • METHODS AND RESULTS We performed a low-coverage 454 next-generation sequencing of enriched genomic fragments derived from one individual to generate a massive library of contigs...
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ژورنال
عنوان ژورنال: Applications in Plant Sciences
سال: 2015
ISSN: 2168-0450
DOI: 10.3732/apps.1500036